identifiler plus user guide

These items should never leave the PCR setup work area. DISCLAIMER: LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON- INFRINGEMENT. Version 1.4 Oct 2012 From cells to cdna in one tube, Essentials of Real Time PCR. Start the run. 1. G Revision Date September. PDF Table of Contents - Washington, D.C. Although each of these dyes emits its maximum fluorescence at a different wavelength, there is some overlap in the emission spectra between the dyes (Figure 3). al. Troubleshooting Sequencing Data Troubleshooting Sequencing Data No recognizable sequence (see page 7-10) Insufficient Quantitate the DNA. This is a preview of subscription content, access via your institution. PubMed Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Do not refreeze the reagents. Pipette the required volumes of components into an appropriately sized polypropylene tube. Ambion and Applied Biosystems products are for Research Use Only. Instrument and software compatibility Instrument 3500/3500xL Data Collection Software 3500 Series Data Collection Software v1.0 or later Analysis software GeneMapper ID-X Software v1.2 or later 3130/3130xl 3100/3100- Avant 3.0 or later 1.1 (3100) GeneMapper ID Software v3.2.1 and 1.0 (3100-Avant) 2.0 GeneMapper ID-X Software v1.0.1 or later We conducted validation studies for the AmpFlSTR Identifiler Plus Kit using this configuration. Component Description 50 Volume (A26182) 100 Volume (A26364) 200X Volume ( ) 1000X Volume ( ) Storage AmpFlSTR Identifiler Plus Master Mix AmpFlSTR Control DNA 9947A AmpFlSTR Identifiler Plus Primer Set AmpFlSTR Identifiler Plus Allelic Ladder Contains enzyme, salts, dntps, carrier protein, and 0.04% sodium azide. Jiang B, Qu W, Wang F, Zhang L, Rong H, Li J, Wen DIAMETER, Zeye MMJ, He W, Wang C, Link H, Zhang T, Jin C, Chen L, Liu Y, Cai J, Zha L. Int J Legal Med. and E. japonicus) DNA detection by. J Forensic Sci 53(4):838. While enhancing throughput with asingle amplification is a key feature ofthe Identifiler Kit, other factors areimportant to the human identificationlaboratory, including robustness andreliability of the PCR amplification.The AmpFlSTR Identifiler Kit wasdesigned as a single amplification withthese important design goals in mind: Use with the optimum 1.0 ng DNA input amount in a maximum input volume of 10 l. Cells contain DNA. PDF Amplification Kits GlobalFiler and GlobalFiler IQC PCR II. TRADEMARKS All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified Thermo Fisher Scientific, Inc. All rights reserved. Springer, Singapore. See the reference cited in the following table for details about these kits. GeneAmp PCR System 9700 with the Silver 96-Well Block GeneAmp PCR System 9700 with the Gold-plated Silver 96-Well Block Veriti 96-Well Thermal Cycler ProFlex PCR System 24 AmpFlSTR Identifiler Plus User Guide, 25 Required user-supplied reagents Required user-supplied reagents In addition to the AmpFlSTR Identifiler Plus Kit reagents, the use of low TE buffer (10 mm Tris, 0.1 mm EDTA, ph 8.0) is recommended. Julio MJ et al (2013), Next generation STR Genotyping kits for Forensic applications, Forensic DNA Analysis- Current Practices & Emerging Technology, 1st Edition, ISBN: 9780429254338, CRC Press. Alleles that are not in the AmpFlSTR Allelic Ladders do exist. Not. About Sequence Detection Chemistries, STA DARD OPERATI G PROCEDURE FOR THE DETECTIO OF AFRICA SWI E FEVER VIRUS (ASFV) BY CO VE TIO AL POLYMERASE CHAI REACTIO (PCR), Easy Collection and Extraction of BioSamples Ahlstrom GenCollect Ahlstrom GenCollect Color. Reagent Volume per reaction (l) GeneScan 500 LIZ Size Standard 0.5 Hi-Di Formamide 24.5 Note: Include additional samples in your calculations to provide excess volume for the loss that occurs during reagent transfers. Two additional loci, D2S1338 and D19S433, are included. The AmpF'STRIdentifiler Plus PCR AmplificationKit has been validated according to the FBINational Standards and Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines.The validation results support the use of the AmpF'STRIdentifiler Plus PCR Amplification Kit for human identity and parentage testing. Highlights. 310 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, GeneAmp 9600, GeneAmp 9700, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneScan 500 LIZ Size Standard, Matrix Standard Set DS-33, D21S11, CSF1PO, vWA, D8S1179, TH01, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D7S820, D13S317, D19S433, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, AmpFLSTR Identifiler Plus PCR Amplification Kit, For licensing and limited use restrictions visit, Spectroscopy, Elemental and Isotope Analysis. The AmpFlSTR Identifiler Plus Kit contains all the necessary reagents for the amplification of human genomic DNA. 360 Master Mix. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition. eCollection 2021. Product description The AmpFlSTR Identifiler Plus Kit uses the same primer sequences as the earlier generation AmpFlSTR Identifiler Kit. The Identifiler Plus method involved use of an in-house extraction buffer prepared from reagents readily available in the laboratory. 38 AmpFlSTR Identifiler Plus User Guide, 39 Section instrument Section instrument Set up the 310 instrument for electrophoresis Reagents and parts Table 3 on page 18 lists the required materials not supplied with the AmpFlSTR Identifiler Plus Kit. Pull-out chapters This guide is designed to allow users to pull out chapters 2, 3, and 4. Zhang ROENTGEN, Tan Y, Wang L, Jian H, Zhu BOUND, Xiao YTTRIUM, Tan M, Xue J, Yang F, Liang W. Front Genet. Evaluation of the Autosomal STR Markers and Kits | SpringerLink 2. GeneAmp PCR System 9700 The GeneAmp PCR System 9700 fits your lab bench, your applications and your budget. B September 2010 Remove patent numbers. 0174 Version 20040915 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. Protect the primer set from light when not in use. No. Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Epub 2015 Arp 23. To prepare low TE buffer: 1. For safety information, see the ABI PRISM 310 Genetic Analyzer User s Manual (PN 903565 or 4317588). PubMed :4440211, Applied Biosystem, User Guide, Verifiler Direct PCR amplification kit (2013) catalog number 4479567, Publication No 4479552, Applied Biosystem, User Guide, Verifiler express PCR amplification kit (2017) Catalog numbers A32014, A32070, A33032, Publication No 100043588, Applied Biosystem, User Guide, Verifiler Plus PCR amplification kit (2020) Catalog number A35495, Publication No MAN0017493, Applied Biosystem, Westen AA et al (2014) Comparing six commercial autosomal STR kits in a large Dutch population sample. PDF J Forensic Sci, TECHNICAL NOTE CRIMINALISTICS - NFSTC A Note: For safety and biohazard guidelines, Procedures For DNA Sequencing Plant-Microbe Genomics Facility (PMGF) Ohio State University 420 Biological Sciences Building 484 W. 12th Ave., Columbus OH 43210 Telephone: 614/247-6204 FAX: 614/292-6337, Components RT31-020 20 rxns RT31-050 50 rxns RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 l 2 x 50 l 5 x 40 l 2x RT Master Mix (2) 200 l 2 x 250 l 5 x 200 l RNase H (E. coli) 20 l 2 x 25 l, Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013, UltraClean PCR Clean-Up Kit Catalog No. How it works: The Quantifiler Duo DNA Quantification Kit consists of targetspecific and internal control 5' nuclease assays. GeneScan 500 LIZ Size Standard Standard used for obtaining sizing results. PROTOCOL BigDye Direct Cycle Sequencing Kit For Research Use Only. Not for use in diagnostic procedures. BigDye Direct Cycle Sequencing Kit, NimbleGen DNA Methylation Microarrays and Services, Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit, Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. Mix together: 10 ml of 1 M Tris-HCl, ph ml of 0.5 M EDTA, ph ml glass-distilled or deionized water Note: Adjust the volumes accordingly for specific needs. Pull-out chapters This guide is designed to allow users to pull out chapters 2, 3, and 4. Epub 2021 Oct 15. For CODIS purposes, profile reported as 8, 8. To maximize the consistency of quantification results, genomic targets were selected with conserved primer- and probe-binding sites within individual genomes and also with minimal copy number variability between different individuals and population groups. Into each well of a MicroAmp Optical 96-Well Reaction Plate, add: 9L of the formamide:size standard mixture 1L of PCR product or allelic ladder Note: For blank wells, add 10 l of Hi-Di Formamide. Prepare reagents. . Am J Hum Genet 55:175189, CAS PubMedGoogle Scholar, School of Forensic Science National Forensic Sciences University, Delhi Campus, New Delhi, India, DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India, Laboratory of Genetic Identification Department of Legal Medicine, Toxicology and Physical Anthropology Faculty of Medicine University of Granada, Granada, Spain. Positive control Add 10 l of 9947A control DNA (0.1 ng/l). Contains 0.10 ng/l human female 9947A DNA in 0.05% sodium azide and buffer. Thaw the AmpFlSTR Identifiler Plus Kit Master Mix and the AmpFlSTR Identifiler Plus Kit Primer Set, then vortex 3 seconds and centrifuge briefly before opening the tubes. Butler JM (2018) Short tandem repeat typing technologies used in human identity testing. One .gov measures its official. The combination of a five-dye fluorescent system and the inclusion of non-nucleotide linkers allows for simultaneous amplification and efficient separation of the 15 STR loci and Amelogenin during automated DNA fragment analysis. Verifiler direct PCR amplification kit for paternity testing, 78-poster verifiler, Life Technology Corporation. Applied markets-Human identification, Tanya C (2015) Identification of variant alleles at CODIS STR loci in different populations of India. AmpFlSTR Identifiler Plus PCR Amplification Kit User`s Guide. For Research Use Only. Download to EndNote/RefMan (RIS) Metadata Show full item record. ABSTRACT. IMPORTANT! sequencing. 2021 Mar;135(2):431-440. doi: 10.1007/s00414-020-02490-y. 1 USER GUIDE AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide Publication Number Revision F For Forensic or Paternity Use Only. Development and validation of novel 8-dye short tandem repeat multiplex system fork forensic applications. A Revision Date April 2011 For Research Use Only. Federal government websites often end in .gov or .mil. Start the electrophoresis run. In addition, the Quantifiler HP and Trio Kits each contain a Large Autosomal target with a longer amplicon (>200 bases) to aid in determining if a DNA sample is degraded. The combination of the 15 loci are consistent with several worldwide database recommendations. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. The AmpFlSTR Identifiler Plus Allelic Ladder contains most of the alleles reported for the 15 autosomal loci. Not intends required whatever animal conversely human therapeutic or diagnostic use. PLoS One. IMPORTANT! Protocol Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR Copyright 2008, 2010 Applied Biosystems. Instruments Refer to Instrument and software overview on page 15 for a list of compatible instruments. Installation Options. Everywhere 3 Untranslated Region (3 UTR) 150 tests, Table of Contents. Touch DNA and DNA Recovery 1 2 What is the link between cell biology & forensic science? Amplified DNA, AmpFlSTR Identifiler Plus Allelic Ladder, and GeneScan 500 LIZ Size Standard should also be protected from light. USER GUIDE For Forensic or Paternity Use Only. Materials and Methods All samples were analyzed using the following: Amplification: Identifiler Direct or Identifiler Plus on GenaAmp PCR System 9700 Information in this document is subject to change without notice. 2017;1492:1-15. doi: 10.1007/978-1-4939-6442-0_1. If you are storing the DNA Then place at < 2 weeks 2 to 8 C > 2 weeks 15 to 25 C IMPORTANT! AmpFlSTR Identifiler Plus PCR Amplification Kit User Guide H. Miller Coyle, Terra PCR Direct Polymerase Mix User Manual, Introduction. The four dyes used in the AmpFlSTR Identifiler Plus PCR Amplification Kit to label samples are 6-FAM, VIC, NED, and PET dyes. AmpFLSTR Identifiler Plus PCR Amplification Kit Provided by the Springer Nature SharedIt content-sharing initiative, Over 10 million scientific documents at your fingertips, Not logged in Not intended for any animal or human therapeutic or diagnostic use. Search Res 14(4):1484714856. 8. QUICK REFERENCE Applied Biosystems 3500/3500xL Genetic Analyzer with 3500 Series Data Collection Software 3 Catalog Number 4405186, 4405187 Pub. The AmpFlSTR Identifiler Plus Kit delivers a 16-locus multiplex with the same power of discrimination as, better sensitivity than, and better robustness than the earlier generation of the AmpFlSTR Identifiler Kit. Handbook of DNA Profiling pp 151184Cite as. Scientifica (Cairo). Butler JM (2015) STR Profiles: Multiplex PCR, Tri-alleles, Amelogenin and partial profiles. FP401, Biomolecular Core Facility AI Dupont Hospital for Children, Rockland Center One, Room 214 Core: (302) 651-6712, Office: (302) 651-6707, mbcore@nemours.org Katia Sol-Church, Ph.D., Director Jennifer Frenck, 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System), The GeneAmp PCR System 9700 Results you can trust. The Quantifiler Trio kit and the HID v1.2 software are designed for use with the Applied Biosystems 7500 Real-Time PCR System. A small punch disc of the card containing the sample can be placed directly into an amplification tube, purified, and amplified, without transferring the disc. 8004-A30 (30 rxns) Reagents Materials are provided for 30 cdna synthesis, Agilent High Sensitivity DNA Kit Guide Agilent High Sensitivity DNA Agilent Technologies Notices Agilent Technologies, Inc. 2009, 2013 No part of this manual may be reproduced in any form or by any means, Product Bulletin AmpliTaq Gold 360 Master Mix and 360 DNA Polymerase AmpliTaq Gold 360 Master Mix AmpliTaq Gold 360 DNA Polymerase 360 Coverage for a Full Range of Targets AmpliTaq Gold 360 Master Mix, ab185915 Protein Sumoylation Assay Ultra Kit Instructions for Use For the measuring in vivo protein sumoylation in various samples This product is for research use only and is not intended for diagnostic. (eds) Handbook of DNA Profiling. 9. 4440211). 2. J Forensic Sci. However, it is recommended that each laboratory determine the optimum cycle number based on internal validation studies. Aliquot and autoclave the solutions. For Forensic or Paternity Use Only. Materials Required 5. Safety 4. 9/06 Part# GE181. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. AmpFlSTR Identifiler Plus Allelic Ladder Allelic ladder for accurate characterization of the alleles amplified by the AmpFlSTR Identifiler Plus Kit. 4. Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. These loci are consistent with the AmpFlSTR SGM Plus PCR Amplification Kit. Results you can trust. Forensic Sci Int Genet Suppl 5:e135e137. Dispense 15 l of the reaction mix into each reaction well of a MicroAmp Optical 96-Well Reaction Plate or each MicroAmp tube. Both the averages (27.1 vs 26.4 for Identifiler Plus and IDplex Plus) and the standard deviations (8.7 vs 8.8) of the two kits were very close to each other (p = 0.601). See Table 1 on page 11 for a list of alleles included in the allelic ladder. 2023 Springer Nature Switzerland AG. Your laboratory can use multiple ladder samples in an analysis, provided individual laboratories conduct the appropriate validation studies. IMPORTANT! Developmental operational of the STRscan-17LC tackle: ampere 6 Dye STR builder enhanced stability and ability to detect degraded samples. Applied Biosystems 3730/3730xl DNA Analyzer, SYBR Green Realtime PCR Master Mix -Plus-, AFLP System Analysis Getting Started Guide, RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr), Introduction To Real Time Quantitative PCR (qpcr), Reconstituting and Diluting Primers and TaqMan Probes, MLX BCG Buccal Cell Genomic DNA Extraction Kit. Google Scholar, Ensenberger MG et al (2016) Developmental validation of Powerplex Fusion 6C system. ed. 5. DNA Integrity Number () For the Assessment of Genomic DNA Samples in Real-Time Quantitative PCR (qpcr) Experiments Application Note Nucleic Acid Analysis Author Arunkumar Padmanaban Agilent Technologies, High-Capacity cdna Reverse Transcription Kits For 200 and 1000 Reactions Protocol Copyright 2006,. 2 V Simplified Procedures. Quality Assurance Manual, the accreditation standards under ISO/IEC 17025:2005, and the supplemental standards set by the FSL's accrediting body, as well as the . Time the primer sequences and dye configurations were unchanged, the AmpFSTR() Identifiler() Plus PCR Amplification Kit features einen extended fender formulation and an optimized PCR cycling protocol that growths sensitivity, provides better acceptance toward PCR inhibitors, and improves performance on mixture samples. For details on the procedures, refer to the documents listed in the table. A selection guide for the new generation 6-dye DNA profiling systems
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